A Genome-Wide RNAi Screen to Dissect Centriole Duplication and Centrosome Maturation in Drosophila (original) (raw)
Figure 7
Polo and Cnn Are Interdependent for Their Localisation and Function at Centrosomes
(A) Pictures from the primary screen of S2R+ cells treated with dsRNA against GFP (control), Cnn, and polo. The localisation of Cnn (green), Phospho-histone H3 (red), and DNA (blue) is shown.
(B) S2R+ cells treated with RNAi against GFP, Cnn, and Polo were stained with antibodies against Cnn (green) and Polo (red), and counterstained with Hoechst (blue).
(C) S2R+ cells treated with RNAi against GFP, Cnn, and polo were stained with antibodies against Cnn (green) and “active” Polo (red), and counterstained with Hoechst (blue). Note how the depletion of Cnn disrupts the centrosomal, but not the kinetochore, localisation of Polo, whereas the depletion of Polo disrupts the centrosomal localization of Cnn.
Scale bar in (A) represents 15 μm, in (B and C), it represents 5 μm.