A Morning-Specific Phytohormone Gene Expression Program underlying Rhythmic Plant Growth (original) (raw)
Figure 4
The HUD Motif (CACATG) Confers Diurnal and Circadian Regulation
(A) The HUD motif (CACATG) is overrepresented in promoter of cycling genes expressed (_Z_-score, significance score) at midday under continuous light (red) and dawn under short-day photocycles (black) [19]. The _Z_-score profile is double plotted for visualization purposes. Black dotted line is the _Z_-score significance threshold (p < 0.05).
(B) Phase overrepresentation of the 54 phytohormone genes with one or more HUD in their promoter (500 bp) under short-day photocycles (black) or continuous light (red).
(C) Venn diagram of the 54 phytohormone genes with at least one HUD in their promoter (500 bp) and either the genes that are not growth associated (111 genes, green) or are growth associated (71 genes, blue).
(D) Two independent T3 lines (6 and 30) carrying three tandem HUD repeats fused to LUC and a minimal promoter confer night-specific activity under short-day photocycles (black lines) and evening-specific activity under continuous conditions (red lines). Data represent the average of four to six seedlings from two independent experiments. Error bars represent standard error of the mean.