SARS-Coronavirus Replication Is Supported by a Reticulovesicular Network of Modified Endoplasmic Reticulum (original) (raw)
Figure 6
Immunogold EM of the SARS-CoV Replicase in Infected Cells
SARS-CoV–infected Vero E6 cells were cryofixed at 8 h p.i. and processed for FS and IEM using rabbit antisera (see Materials and Methods). In all images, 15-nm colloidal gold particles conjugated to protein A were used for detection of primary antibodies.
(A and B) Labeling for SARS-CoV nsp3 was mainly found on the electron-dense areas between DMVs, presumably representing CM as most clearly visible in (B).
(C) Immunolabeling for SARS-CoV nsp5 (the viral main protease), which was essentially similar to that for nsp3.
(D) When using an antiserum recognizing SARS-CoV nsp8 (the putative viral primase), the majority of label was again present on CM. However, a small fraction of the nsp8 signal was reproducibly found on the interior of DMVs.
Scale bars represent 250 nm.