BEAF Regulates Cell-Cycle Genes through the Controlled Deposition of H3K9 Methylation Marks into Its Conserved Dual-Core Binding Sites (original) (raw)
Figure 7
BEAF—But Not DREF—Binding Sites Restrict the Deposition of H3K9me3 Marks in Dual-Cores
(A) Schematic representation of Dcore_38D mutated for its DREF (dre-mut; see Figure 5) or BEAF (beaf-mut) binding sites. The mutated CGATAs (arrowheads) are represented by the *.
(B) Quantitative PCR analysis following ChIP with mock control or with anti-H3K9me3 antibodies of both transfected wild-type (WT) or mutated constructs (A) and endogenous ("endo") Dcore_38D from the same batch of cells. The _y_-axis shows the percentage of material precipitated from the inputs. For transfected constructs, the percentage was normalized to the DNA copy-number in the input (for details, see Materials and Methods). Experimental error is denoted by the differentially colored (gray) portion at the top of each bar.