Comparative Functional Analysis of the Caenorhabditis elegans and Drosophila melanogaster Proteomes (original) (raw)
Figure 1
Workflow of the C. elegans Proteome Analysis
Proteins and peptides were isolated from whole worm or egg homogenates, and separated biochemically. Peptides were identified by μLC-ESI-MS/MS and database searches, and validated using the Trans-Proteomic Pipeline [62]. We detected peptides for 10,631 different gene loci, which corresponds to 54% of the predicted gene loci in WormBase WS140 (19,735 gene loci). For 7,476 gene loci, more than one peptide was identified; for 580 gene loci, a single peptide was identified independently multiple times; for 2,575 gene loci, a single peptide was identified; and 9,104 gene loci were not covered at all.