Commitment to the Regulatory T Cell Lineage Requires CARMA1 in the Thymus but Not in the Periphery (original) (raw)
Figure 2
Positional Cloning of the king Mutation
(A) Percentage of circulating CD4+ T cells expressing Foxp3 in king homozygous and heterozygous mice.
(B) Linkage of the king phenotype to Chromosome 5, using 134 informative microsatellite markers.
(C) Genomic sequence of Carma1 in wild-type and king mice, with corresponding amino acid sequence.
(D) Diagram of the CARMA1 protein (star, king mutation; _α_-helices, indicated by rings). Abbreviations: NORS, no regular secondary structure; SH3, Src homology 3; GUK, guanylate kinase.
(E) Western blot analysis of CARMA1 expression in thymus, spleen, and lymph node lysates.
(F) CARMA1 expression in CD4+CD8−CD3ε+ (CD4 SP) and CD4−CD8+CD3ε+ (CD8 SP) sorted thymocytes.