Distinct Dynamics of Endocytic Clathrin-Coated Pits and Coated Plaques (original) (raw)
Figure 5
Recruitment of cortactin, Arp2/3, and dynamin into clathrin-coated pits and plaques.
Spinning disk fluorescence intensity profiles obtained from time-series images of clathrin assemblies forming at the adherent (bottom) surface of Swiss 3T3 fibroblasts stably expressing Clathrin LCa-DsRed and transiently expressing (A) cortactin-EGFP, (B) Arp3-EGFP, and (C) dynamin2-EGFP. Fluorescence images for each set of these proteins were simultaneously acquired with a beam splitter using 200-ms exposures obtained every 5 s. The points represent averages of complete datasets, normalized to the maximum intensity and to the lifetime of the object. Each point represents average plus or minus standard deviation. N is the number of coated pits and plaques analyzed. The maximum recruitments of clathrin LCa-DsRed, cortactin-EGFP, Arp3-EGFP, or dynamin2-EGFP by plaques are statistically higher (p<0.0001) than recruitment by pits.