Distinct Dynamics of Endocytic Clathrin-Coated Pits and Coated Plaques (original) (raw)
Figure 7
Relative positions of clathrin, AP-2, and epsin in coated pits and plaques.
(A) Models representing the distribution of clathrin, AP-2, and epsin during the formation of a canonical clathrin-coated pit or a clathrin-coated plaque. (B) Difference in the axial positions of clathrin Tomato-LCa or clathrin LCa-DsRed with respect to AP-2 σ2-EGFP (Δ_z_, red) or to epsin1-EGFP (Δ_z_, blue) determined for individual pits or plaques selected for DiNa analysis in U373 astrocytes (52 pits and 40 plaques) or Swiss 3T3 fibroblasts (49 pits and 45 plaques) cells. The difference of axial position within each pit is plotted as a function of time; to facilitate the comparison between pits of different lifetimes, the time component is normalized as a fraction of each lifetime. DiNa results were obtained from data acquired three Swiss and three U373 astrocytes. The axial separations become statistically significant (p<0.0001) when the pits reach a maturity of 50% or more. (C) Difference in the axial position of clathrin Tomato-LCa with respect to AP-2 σ2-EGFP (Δ_z_, red) or to epsin1-EGFP (Δ_z_, blue) determined for 15 and 14 individual pits selected for DiNa analysis in BSC1 cells, respectively. Similar DiNa results were obtained from data acquired in three additional Swiss and three additional astrocytes (unpublished data). Error bars in (B) and (C) indicate plus or minus the standard deviation.