The Mechanochemistry of Endocytosis (original) (raw)
Figure 1
Endocytic dynamics in budding yeast.
(A) Timelines for endocytic protein recruitment as determined by multicolor fluorescence microscopy analysis. Sla1p, which is an endocytic adaptor protein, represents the endocytic coat. Abp1p is an actin-binding protein and faithfully reports on actin dynamics. Sjl2p is the yeast synaptojanin that hydrolyzes PIP2. PIP2 represents the lipid module and is believed to be the recruitment signal for many endocytic proteins. Rvs167p, yeast Amphiphysin, contains a BAR domain capable of sensing/binding curved membranes and deforming membranes. (Sla1 and Abp1 data are from [8], Sjl2 data are from [11], Rvs167 data are determined in this work from six individual patches in cells expressing Rvs167-GFP and aligned to the relative timing of Sjl2 appearance.) (B) Spatial profiles of endocytic membrane and the key endocytic proteins as revealed by EM [15].