Cadherin-2 Controls Directional Chain Migration of Cerebellar Granule Neurons (original) (raw)
Figure 11
Impaired terminal differentiation of migrating Cadherin-2 deficient GCs.
(A–F) Dorsal views of WT (A, C, E) and _pac_−/−R cerebella (B, D, F). ISH for the granule cell differentiation markers neuroD (A, B, 3dpf), vglut1 (C, D, 4dpf), and gabαRa6 (E, F, 6dpf). NeuroD expression in _pac_−/−R cerebella appears scattered, likely resulting from randomly migrating GCs. However, the strength of expression is similar to WT. Vglut1 expression is strongly reduced in _pac_−/−R and expression of the terminal granule cell differentiation marker gabaRAα6 is almost absent in _pac_−/−R cerebella (black arrows depict residual patches of expression in ventro-lateral cerebellar regions). cb, cerebellum; ot, optic tectum.