Sequestration of the Aβ Peptide Prevents Toxicity and Promotes Degradation In Vivo (original) (raw)
Figure 1
Inhibition of neurotoxicity measured as lifespan of transgenic Drosophila.
Each curve represents 100 flies divided equally into groups of 10. Expression of all Aβ peptides and Affibody proteins was under the control of the UAS-GAL4 system. In these experiments, expression was driven throughout the CNS by the elavc155-GAL4 driver line. Survival assays were performed to quantify the degree of neurodegeneration when each different combination of Aβ peptide and Affibody proteins or Z domain control was expressed in the CNS. (A) Aβ42e22g median lifespan = 9 (±0.5) days; Aβ42e22g + ZAβ3 = 20 (±0.2) days, p<0.001 versus Aβ42e22g alone; Aβ42e22g + (ZAβ3)2 = 31 (±0.8) days, p<0.001 versus Aβ42e22g alone. (B) Aβ42 median lifespan = 28 (±0.4) days; Aβ42 + ZAβ3 = 32 (±0.7) days, p<0.001 versus Aβ42 alone; Aβ42 + (ZAβ3)2 = 40 (±1.2) days, p<0.001 versus Aβ42 alone. (C) Aβ40 median lifespan = 38 (±2); Aβ40 + ZAβ3 = 41 (±2) days; Aβ40 + (ZAβ3)2 = 38 (±2) days. (D) Control experiment: lifespan of flies expressing only the Z domain, ZAβ3, or (ZAβ3)2 and non-transgenic flies (wild-type). Median lifespan of wild-type flies = 38 (±1.8) days. Complete survival statistics are shown in Table S1.