A Complete Developmental Sequence of a Drosophila Neuronal Lineage as Revealed by Twin-Spot MARCM (original) (raw)
Figure 5
Distinct cell counts in different adPN types.
Twin-spot clones labeled with GAL4-GH146 (A–G) or acj6-GAL4 (K–M). Upper panels: composite confocal images of sister clones in the AL; lower panels: single focal sections showing no innervation of the magenta glomeruli by the green NB clones, indicating clones derived during birth of the last sibling of the preceding adPN type. The clones shown in (A) and illustrated in (H) reveal the adPN NB makes five VA1lm-targeting PNs following derivation of the last DM6-targeting PN. Illustrations of lineage development for additional twin-spot clones are shown in (I), (J), and (N) to (P). Invariant cell counts were obtained for the majority of NB clones paired with the last sibling of the preceding adPN type (see Tables S3 and S4). These support production of a fixed number of neurons for each multi-cellular adPN type, as summarized in the bottom.