A High Precision Survey of the Molecular Dynamics of Mammalian Clathrin-Mediated Endocytosis (original) (raw)
Figure 3
Individual Dyn1-mCherry recruitment events were variable, but the average Dyn1-mCherry recruitment signature was stable.
(A and B) Natural variation of Dyn1-mCherry recruitment to sites of scission. (Ai–Avi) Consecutive images of TfR7 (top), TfR5 (middle), and Dyn1-mCherry (bottom) movies centred on scission events detected in the TfR5 movies. The data are from the cell shown in Figure 2A. (Bi–Bvi) Quantification of fluorescence for TfR5 (light green curves), TfR7 (dark green curves), and Dyn1 (red curves) images for the corresponding events shown in (A). Vertical blue lines indicate t = 0 s, and black horizontal lines indicate zero fluorescence. Horizontal scale bar corresponds to 20 s, fluorescence values as indicated. Dots correspond to frames shown in (A). (C) The full set of Dyn1-mCherry fluorescence traces were normalised and overlaid as a cloud plot (see Materials and Methods). Red indicates higher data density, blue, lower density, and black, background. The average fluorescence recruitment trace is indicated by a white line. (D) Replicate Dyn1-mCherry recruitment signatures for either human (Hs) or mouse (Mm) Dyn1-mCherry. au, arbitrary units; WT, wild type.