Mesenchymal Transition and Dissemination of Cancer Cells Is Driven by Myeloid-Derived Suppressor Cells Infiltrating the Primary Tumor (original) (raw)
Figure 7
Inducers of EMT.
(A) Expression of Hgf, Egf, and Tgfb1 genes in RETAAD tumors analyzed by qRT-PCR. Hgf and Tgfb1 are preferentially expressed in PMN-MDSC, while Egf is preferentially expressed in tumor cells. Data are from four individual experiments using sorted fractions of PMN-MDSC and melanoma cells. Bars represent mean ± SD. (B) NBT-II cells (100 cells per well) were plated for 4 d to allow for colony growth and were pre-treated for 24 h with inhibitors before the addition of PMN-MDSC. After co-culture with PMN-MDSCs in the presence of inhibitors, cells were fixed and stained with anti-rat desmoplakin. Green, Desmoplakin; red, H2b (nuclear stain). PD153035 – EGFR inhibitor, JNJ38877605 – c-met (HGFR) inhibitor, and SB525334 – TGF-βR1 inhibitor. Images are representative of three independent experiments.