USP8 Promotes Smoothened Signaling by Preventing Its Ubiquitination and Changing Its Subcellular Localization (original) (raw)
Figure 7
USP8 promotes Smo signaling activity.
(A–B) Wild-type wing discs were stained with anti-β-Gal and anti-Ptc antibodies to show dpp-lacZ and endogenous Ptc expression, respectively. (C–L) Wing discs expressing GFP-Smo alone (C–D), Flag-USP8 alone (E–F), GFP-Smo and Flag-USP8 together (G–H), HA-USP8NT3 alone (I–J), or GFP-Smo and HA-USP8NT3 together (K–L) by MS1096 Gal4 were stained for dpp-LacZ or Ptc. The red arrows indicate the ectopic expression of either dpp-lacZ or Ptc. (M–M′) A wild-type wing disc was immunostained for ptc-lacZ and En. (N–O′) Wing discs expressing SmoSD123 alone or together with USP8RNAi by MS1096 Gal4 was immunostained for ptc-lacZ and En. Knockdown of USP8 by RNAi attenuated the ectopic _ptc_-lacZ expression (arrow in O) and greatly reduced the ectopic en expression (arrow in O′).