Stochastic Expression of the Interferon-β Gene (original) (raw)
Figure 2
Viral transcription and/or replication are more efficient in IFNβ-producing cells.
(A) qPCR analysis illustrating the relative abundance of viral NP, matrix (M), and L polymerase protein (L) mRNA in sorted IFNβ/YFP MEFs. (B) Western blots showing cytoplasmic distribution of SeV NP protein present in IFNβ-producing and nonproducing cells. (C) qPCR analysis illustrating the relative abundance of SeV DI genome (upper panel), and semi-qRT-PCR analysis illustrating the relative abundance of SeV genomic RNA (lower panel) in sorted IFNβ/YFP MEFs. Reverse transcriptase PCR was carried out to detect viral genomic RNA and host cell β-actin mRNA (control) using gene-specific primers. After 35 cycles (SeV genomic RNA) or 26 cycles (β-actin) of amplification, PCR products were run on a 2% agarose gel. (D) Intracellular staining using SeV antibody and FACS analysis were carried out to determine the correlation between SeV infection and IFNβ expression in IFNβ/YFP homozygous MEFs. IB, immunoblot.