Neurotransmitter-Triggered Transfer of Exosomes Mediates Oligodendrocyte–Neuron Communication (original) (raw)
Figure 3
NMDA and AMPA receptors mediate exosome release.
Quantification of exosome release upon (A) stimulation with glutamate receptor agonists NMDA (100 µM, n = 7), AMPA (100 µM, n = 5), or NMDA and AMPA (n = 4), (B) application of NMDA and AMPA receptor antagonists MK801 (5 µM, n = 9) or NBQX (25 µM, n = 9), or (C) the NMDA receptor co-agonist D-Serine (100 µM, n = 5). (D) Glutamate-stimulated exosome release from NMDA receptor-deficient oligodendrocytes obtained from CNPCre/+*NR1flox/flox mice (n = 6) compared to CNPCre/+ control mice (n = 9). Representative Western blots of PLP in exosome pellets are depicted. Error bars, SEM (n.s., not significant ; * p<0.05; ** p<0.01; Wilcoxon test).