Developmental Changes in the in Vitro Activated Regenerative Activity of Primitive Mammary Epithelial Cells (original) (raw)
Figure 1
Representative frequencies of phenotypes, CFCs, and MRUs in E18.5 fetal and adult mammary tissue.
(A) Representative gating and final FACS profiles of viable subsets of single cells isolated from dissociated E18.5 mammary glands after exclusion of hematopoietic (CD45+ and Ter119+) and endothelial (CD31+) cells. Embedded images are negative controls. (B) Frequency of total cells, CFCs, and MRUs measured in the indicated subsets and in suspended but not further separated fetal mammary (EpCAM+) cells. (C) Representative gating and final FACS profiles of viable subsets of single cells isolated from dissociated 8–12-wk-old adult mammary glands after exclusion of hematopoietic (CD45+ and Ter119+), endothelial (CD31+), and stromal (BP-1+) cells. A fluorescence-minus-one control was used to set the gate used to identify CD61+ cells. (D) Frequency of total cells, CFCs, and MRUs measured in the indicated subsets and in unseparated suspensions of adult mammary epithelial (EpCAM+) cells.