Developmental Changes in the in Vitro Activated Regenerative Activity of Primitive Mammary Epithelial Cells (original) (raw)
Figure 6
Comparison of CFC and MRU outputs in 7-d Matrigel cultures initiated with single fetal or adult mammary cells.
(A) Correlation between the total number of cells retrieved from individually assessed 7-d cultures with the corresponding number of CFCs detected. (B) Distribution of clonal CFC outputs in 7-d cultures initiated with different types of input cells. The dotted line indicates the median values of positive clones: 1,000 for clones derived from basal adult cells (n = 44), 140 for clones from CD61+ adult luminal cells (n = 35), and 13,000 for clones from fetal cells (n = 55). (C) Comparison of MRU outputs determined by LDA in 7-d Matrigel cultures initiated with single adult and fetal mammary cells. The value for fetal cells is significantly higher than either of the adult values (p<0.01, Chi-square p value, pair-wise comparison of stem cell frequencies, ELDA, Table S7).