Gasdermin D mediates the pathogenesis of neonatal-onset multisystem inflammatory disease in mice (original) (raw)
Fig 1
GSDMD cleavage correlates with IL-1β secretion and pyroptosis.
BMMs were isolated from WT, _Gsdmd_−/−, _Nlrp3_−/−, _Casp1_−/−, _Casp11_−/−, NOMID, or NOMID;_Gsdmd_−/− mice and were expanded in vitro in the presence of M-CSF-containing media. BMMs were primed with LPS for 3 hours and treated with nigericin for 30 minutes. (A, D) Western blot analysis of cell lysates. (B, E) IL-1β levels in conditioned media. (C, F) Percent of LDH release in conditioned media. Data are mean ± SEM from experimental triplicates and are representative of at least three independent experiments. The numerical values underlying Fig 1B, 1C, 1E and 1F can be found in S1 Data. ****P < 0.0001. BMM, bone marrow–derived macrophage; Casp, caspase; GSDMD, gasdermin D; IL-1β, interleukin-1β; LDH, lactate dehydrogenase; LPS, lipopolysaccharide; M-CSF, macrophage colony-stimulating factor; NOMID, neonatal-onset multisystem inflammatory disease; WT, wild-type.