Delayed death in the malaria parasite Plasmodium falciparum is caused by disruption of prenylation-dependent intracellular trafficking (original) (raw)
Fig 9
Supplementing ubiquinone-independent yDHODH-expressing transgenic parasites with GGOH does not protect from delayed death in the third IDC after treatment.
Dose-response curve from SYBR-Green susceptibility assay determined 48, 96, and 144 hours post indolmycin treatment, with and without polyprenol (5 μM GGOH) rescue. Assays were performed in parallel with 3D7 and transgenic parasites expressing ubiquinone-independent yDHODH. yDHODH parasites are susceptible to delayed death equivalent to 3D7 (inhibition at 96 hours). GGOH supplementation does not further protect yDHODH parasites in the third IDC (inhibition at 144 hours). Representative of at least three independent experiments. Scatter plots show error bars with mean ± SEM. See S2 Data for numerical data underlying figure. GGOH, geranylgeraniol; IDC, intraerythrocytic developmental cycle; yDHODH, yeast dihydroorotate dehydrogenase.