Simulating Properties of In Vitro Epithelial Cell Morphogenesis (original) (raw)
Figure 6
An Invalid Response from Analogue 1
The sketched screenshots show the induced growth response of a monolayer of cells in simulated surface culture following placement of a second layer of cells above the first. A black space represents free space or a simulated lumen space; a light shaded space represents matrix. All the cells in a stable monolayer in vitro are polarized and so are expected to be relatively unresponsive to the newly added cells. First, a stable monolayer of cells was formed. The simulation was stopped. A layer of cells was placed on top of the monolayer (top: cycle 0). The simulation was restarted. End of cycle 1: many of the added cells have “died;” growth and development of cells in the original monolayer have been stimulated; encroachment of cells into the matrix below has started. Cycle 2: remodeling is under way. Cycle 15: after considerable remodeling, a stable structure along with several inverted cysts has formed.