Simulating Properties of In Vitro Epithelial Cell Morphogenesis (original) (raw)
Figure 8
Retention of Pcell Status
(A) Given a pcell precondition, if three conditions in sequence are also met, as illustrated by the decisional flow diagram, then pcell status is retained. Otherwise, the pcell transitions to cell.
(B) Sketched are sequential screen shots taken during a typical Analogue 2 simulation for simulated embedded culture. Spheres represent cells and pcells; pcells are shaded darker. A black space represents free space or a simulated lumen space; a light shaded space represents matrix. The first evidence of pcells is at the end of simulation cycle 5. Cycle 8: a stable structure has formed; only pcells are present.
(C) An example of an Analogue 2 outcome from each of the four simulated environments, comparable to the Analogue 1 outcome in Figure 5. The status of the developing structures in the four simulated environments at simulation cycle 2 or 5 is shown. Each contains a mixture of cells and pcells: a: a developing cyst in simulated embedded culture; b: a cyst in simulated suspension culture; c: monolayer formation in simulated surface culture; and d: lumen formation in simulated overlay culture.