Simulating Properties of In Vitro Epithelial Cell Morphogenesis (original) (raw)
Figure 10
Analogue 2: Comparisons of In Silico and In Vitro Results
Comparisons are presented of data from experiments in silico (n = 50), using Analogue 2, and in vitro data. A mean cell division time of 12.0 h is assumed.
(A) Cell numbers per cyst in simulated embedded culture. In vitro cell number per cyst data was adapted from the cyst size time series in embedded culture provided in Figure 9 in [7], assuming an EC diameter of 10 μm and spherical hollow cysts. The bars indicate one standard error of the mean.
(B) Average division event and death event counts at each simulation cycle.
(C) The frequency distribution of cell numbers in vitro per cyst cross section after ten days in embedded culture are compared with the frequency distribution of cell numbers per cyst cross section after 12 simulation cycles; black bars: simulation results; white bars: in vitro measurements. The former (in vitro) dataset was obtained by measuring MDCK cyst sizes at day 10 from Figure 2A in [24] (n = 44), assuming an EC diameter of 10 μm and spherical hollow cysts. The latter dataset was the result of n = 1,000 simulations. The insert shows the box plots for both datasets. The box centerline designates the median; the box designates the first through third quartiles; and the whiskers represent the 10% and 90% quantiles.