Posttranscriptional Expression Regulation: What Determines Translation Rates? (original) (raw)
Figure 1
Correlation of Protein Abundance and Different Translation Rates
Shows Spearman rank correlation (rs) of TA versus reference protein abundance; error bars indicate ± one standard deviation based on random subsamples. _TA_1 = mRNA × ribocc × ribden; _TA_2 = _TA_1 × CAI; _TA_3 assumes Michaelis–Menten kinetics for the TA (Equation 3). The tRNA–AIs were calculated as described in Methods; tRNA–AI_p indicates the codon–tRNA assignment according to [42] and tRNA–AI _c the assignment according to Crick's wobble rules [43]. All correlations are based on 4,123 ORFs. Accounting for tRNA–AI_p slightly improves correlations compared with TA1 alone. However, TA2 (with CAI) performs better. Overall, considering saturation, (TA3) gave the best results.