Confidence-based Somatic Mutation Evaluation and Prioritization (original) (raw)
Figure 4
Genome browser screen shot for triplicate black6 and B16 samples and associated Sanger sequencing traces for the black6 and B16 DNA for both a true positive (chr4:155261079) and a false positive (chr4:151534480) mutation call.
Both mutations are predicted by GATK, SomaticSNiPer and SAMtools. The mean coverage is 54 (true positive) and 10 (false positive), respectively. Only four reads are shown for visual clarity. The red box marks the sample, in which the three mutation callers wrongly detected a SNV.