Temporal Expression-based Analysis of Metabolism (original) (raw)
Figure 1
Workflow for integrating multiple data types with TEAM.
TEAM integrates three types of experimental data: starting media composition, expression data, and biomass data. Pre-calculations include normalization of the gene expression data, interpolation of all data sets, and calculation of gene penalties based on the gene expression data. For a given time interval, TEAM calculates the metabolic flux distribution most consistent with gene expression and biomass data. It applies this result to update media conditions for the subsequent time interval.