Transcriptome Profiling of Giardia intestinalis Using Strand-specific RNA-Seq (original) (raw)
Figure 2
A non-transcribed region on chromosome 5.
(A) Correlation of RNA-seq and RT-qPCR gene expression measurements for 49 genes in WB. Black dots are genes. The x- and y-axes show log10 FPKM and −log10 Ct (cycle threshold). Values were incremented by 1 before log10-transformation. Each RT-qPCR reaction was performed in triplicates, and the average Ct was used. The blue line is the linear regression (y = 0.07008x-1.50276). Included genes (prefix GL50803_): 7766, 9662, 6744, 7760, 112103, 11654, 11118, 2661, 24321, 17121, 17585, 14993, 16924, 13272, 6564, 5800, 3367, 17570, 16343, 93548, 11540, 5435, 15000, 21423, 10297, 114210, 86681, 7573, 7715, 102438, 7243, 16438, 17291, 1903, 17495, 102978, 11642, 17539, 90575, 32674, 13091, 137688, 3666, 25075, 16690, 2633, 92664, 13627, 4431. (B) Sliding window analysis of RNA-seq coverage on scaffold CH991767 (part of chromosome 5; WB). Analyzed windows were 500 bp wide and not overlapping. (X-axis) Position along the genomic segment (start position of the analyzed window). (Y-axis) RNA-seq depth in the window on a logarithmic scale. Drop in the RNA-seq coverage is seen in positions 1,340,000 to 1,381,000.