Transcriptome Profiling of Giardia intestinalis Using Strand-specific RNA-Seq (original) (raw)
Figure 5
Polyadenylation sites.
(A) Histogram of clustered polyadenylation sites (PACs) and their normalized position on ORFs. On the x-axis, 0 and 1 refer to the first and last base of the ORF. Only polyadenylation sites of the sense direction with respect to the ORF are shown. The y-axis shows the frequency. The data are from the WB isolate. (B) Bar-plot of the number of sense PACs per ORF. (C) Example of alternative polyadenylation of the gene encoding 3-hydroxy-3-methylglutaryl-coenzyme A reductase (GL50803_7573). Arrows indicate locations of the polyA site. Blue arrows indicate polyA sites of the same direction as the genes, and the orange arrow indicates a polyA site of the reversed strand. Numbers of supporting reads (polyA tags) are shown on top of the arrows. The polyA sites are separated by 275 bp. (D) Histogram of 3′ UTR length (nt) inferred from mapped polyA sites. Only the WB isolate is shown. (E) Relationship between gene expression signal (GES; log10 FPKM) and 3′ UTR length. The y-axis shows the log10 GES and x-axis shows the 3′ UTR length (nt). Only 3′ UTRs <500 nt are plotted. (F) Nucleotide length differences between orthologous 3′ untranslated regions.