Treatment of experimental data on the binding of ArfGAP1 to liposomes of different lipid compositions.
(A) The fraction of bound ArfGAP1 to liposomes of different lipid composition as a function of the estimated monolayer spontaneous curvature , as taken from the experimental study [29] (see Table 2). (B) The relative binding constant as a function of the monolayer spontaneous curvature , as taken from the experimental study [29] (circles), and a comparison with a fit using our model, for an insertion length of and a depth of insertion of (solid line). (C) The relative binding constant numerically computed for laterally coupled symmetric monolayers as a function of the monolayer spontaneous curvature, , for the insertion cross-sectional radius , the insertion depth , and different insertion lengths (, solid line; , dashed line; and , dotted line). (D) The same quantities as in (C) plotted for a long insertion embedded to three different depths (, solid line; , dashed line; and , dotted line). (E) The optimal insertion length, L, as a function of the insertion embedding, d, that best fits the experimental results presented in Table 2 for ArfGAP1 (solid line). The shaded region represents the estimated range of ArfGAP1 insertion lengths.