Construction and Validation of a Regulatory Network for Pluripotency and Self-Renewal of Mouse Embryonic Stem Cells (original) (raw)
Figure 1
Signed and directed network extracted from ChIP-seq and knockdown or over-expression followed by genome-wide expression.
Edges are established where there is evidence for transcription factor binding to the gene proximal region and also change in expression after knockdown or over-expression. The 15 pluripotency nodes are color coded in light blue, and the lineage markers are color coded for the four major early differentiation lineages. Red diamond-heads denote inhibition and black arrowheads denote activation. Dashed lines connect pluripotency regulators to lineage markers and solid arrows connect pluripotency regulators to other pluripotency regulators.