Glucocorticoid Receptor-Dependent Gene Regulatory Networks (original) (raw)
Figure 1
Experimental Paradigm for Orthogonal Analysis of Glucocorticoid Receptor Targets
Treatment mice were fasted overnight, then given intraperitoneal injections with dexamethasone. Treatment and control liver lobes were split in half and processed for both RNA and chromatin. RNA was subjected to microarray analysis using the PancChip 5.0 cDNA microarray, which contains over 13,000 transcripts. Location analysis was performed by immunoprecipitating with antiserum raised against the GR. ChIP material was amplified, fluorescently labeled, and hybridized against sheared genomic DNA using the Mouse PromoterChip BCBC-3.0 promoter microarray, which contains approximately 7,000 genomic promoter elements.