The Notch Ligand JAG1 Is Required for Sensory Progenitor Development in the Mammalian Inner Ear (original) (raw)
Figure 7
Early Analysis of the Patterns of Differentiation in the Jag1-cko Cochlea Indicates the Defects Are Caused by a Failure in the Formation of Sensory Cells and Not Subsequent Degeneration
Lectin staining of whole-mount cochlea at E16.5.
(A) Normal patterning in wild-type control cochlea. GER, greater epithelial ridge.
(B) Both the basal and middle portions of the cochlea are shown, although because it is much longer in the control (A), the very basal portion of the cochlea has been removed. Note the lack of hair cells in the basal portion of the Jag1-cko cochlea.
(C–H) Boxed-in areas of (A) and (B) are shown at higher magnification in (C) and (D). Arrows in (D) indicate the abnormal patches of hair cells also observed at E18.5. Similarly, the boxed-in regions of (E) and (F) are shown at higher magnification in (G) and (H), demonstrating the few hair cells that are just beginning to differentiate in this region in both the control and the mutant (arrowheads). Scale bars = 500 μm for the corresponding panels.