Changes in Gene Expression Foreshadow Diet-Induced Obesity in Genetically Identical Mice (original) (raw)
Figure 2
Stability of the Adiposity Phenotype in B6 Mice
Male mice (n = 107) were fed a low-fat chow diet (Picolab 5053) from weaning to 8 wk of age, a high-fat diet (Research Diets D12331) from 8–14 wk, then the low-fat chow diet, restricted to 80% of the amount consumed from wk 7–8, during wk 15 and16, and finally, the high-fat diet for wk 17–22. Mice were weighed weekly except during the food restriction period when body weights were measured daily until they had stabilized under these conditions. Food intake was measured weekly starting from wk 7. At the end of wk 8, 14, 16, and 22, the body composition of each mouse was analyzed by NMR. The body weight curves of mice at the upper and lower 10% of the frequency distribution at 22 wk of age are plotted in red and blue.