Genetic Regulation of Unsaturated Fatty Acid Composition in C. elegans (original) (raw)
Figure 7
Effects of nhr-80(RNAi) in the Δ9 Desaturase Mutant Background
(A) Photographs showing adult worms after 4 d of growth on nhr-80(RNAi) and empty vector control bacteria. The fat-6 mutants grown on nhr-80(RNAi) are thin, pale, and produce no viable progeny.
(B) Relative abundance of 18:0 expressed as a percentage of total fatty acid as determined by gas chromatography analysis. The fat-6 mutants grown on nhr-80(RNAi) (n = 5) accumulate much higher levels than fat-6 mutants grown on control (n = 7) and wild type grown on nhr-80(RNAi) (n = 6). *significant differences from growth on control bacteria, p < 0.01.
(C) Effects of nhr-80 on Δ9 desaturase gene expression in fat-5, fat-6, and fat-7 mutants. QPCR in fat-5, fat-6, fat-7, and wild type for worms grown on empty vector control bacteria (ev) and nhr-80(RNAi) (80i) (n = 6). Values are expressed relative to fat-6 expression in wild-type worms grown on control bacteria. For all graphs error bars represent standard error.