Genome-Wide Analysis of Gene Expression during Early Arabidopsis Flower Development (original) (raw)
Figure 5
Expression Patterns of Four Genes Encoding B3-Domain-Containing Proteins in Early-Stage Wild-Type Flowers
(A) Microarray results: log10-transformed signal intensities at the individual time points are shown for the genes tested.
(B–I) Results of in situ hybridizations. Expression patterns were analyzed in early-stage flowers of wild-type plants. Arrows point to regions of expression. Expression of At2g35310 was first detected throughout the center of young floral buds (B). At later stages, expression was confined to stamen and carpel primordia (C). Expression of At3g53310 was first detected at stage 4 throughout very young stamen primordia (D). At stage 6, expression was observed in stamen, as well as in carpel primordia (E). Expression of At5g57720 was first detected in stage 3 floral buds adjacent to the emerging sepal primordia (F). Its expression at later stages resembled that of At2g35310 (compare panels [G and C]). Weak expression of At3g46770 in stamen and carpel primordia was first observed in stage 7 floral buds (H). At later stages, expression was confined to the margins of the central septum of the gynoecium (I). (C, G, and I) show transverse sections; in all other panels, longitudinal sections are shown. Numbers indicate approximate floral stages.
Scale bars: 30 μm (B, D, and F); 50 μm (E); 100 μm in all others.
ca, carpel; se, sepal; st, stamen.