Inducible and Reversible Clock Gene Expression in Brain Using the tTA System for the Study of Circadian Behavior (original) (raw)
Figure 6
Locomotor Activity Records of Progeny from Scg2::tTA × tetO::Clockwt -HA Matings: Representative Actograms
Actograms of (A) WT, (B) single transgenic _Scg2_-tTA, (C) single transgenic tetO::_Clockwt_-HA, and (D and E) double transgenic Scg2::tTA/tetO::_Clockwt_-HA mice. All double transgenic mice, expressing the Clockwt -HA TG, showed a shortening of circadian period of approximately 1 h, beyond the normal WT values. Mice that carry only a single TG (Scg2::tTA or tetO::_Clock_Δ_19_-HA) showed circadian period range similar to their WT littermates. At the first arrow, all animals were given Dox (10 μg/ml) to turn off transgenically induced CLOCKwt-HA. At the second arrow, Dox was withdrawn to switch back on the _Clockwt_-HA TG. A similar effect on circadian period was observed when Dox was administered and withdrawn for the second time in the same animal.