Unbiased Gene Expression Analysis Implicates the huntingtin Polyglutamine Tract in Extra-mitochondrial Energy Metabolism (original) (raw)
Figure 4
Pathways Common to HD CAG and 3-NP Reveal Decreased Carbohydrate and Lipid Metabolism, with HD CAG-Specific Pathways Reminiscent of huntingtin Normal Function
(A) Venn diagrams summarize the results of GO analysis (biological process), showing the numbers of significantly altered (Increase, Decrease) HD CAG-specific, 3-NP-specific and shared GO terms, with the major categories of pathways listed below.
(B) Venn diagram, statistical criteria, and NTk value-based heatmap summarize the results of sigPathway analysis to distinguish gene sets that were HD CAG specific (Group 1), 3-NP specific (Group 5), or highly significant in both the HD CAG and 3-NP comparisons (Group 3). Groups 2 and 4 were judged marginally significant. High absolute NTk value indicates high significance and +NTk and –NTk denote enrichment in the control group (ST7/7) and the experimental group (ST111/111 or ST7/7+3-NP), respectively. The major categories of enriched gene sets, with the direction of change denoted by arrows, are listed.
(C) A list of the 15 pathways that were found to be common to the HD mutation and 3-NP treatment, as judged significant in both GO and sigPathway analysis. All were enriched in wild-type cells (ST7/7) compared to mutant striatal cells or 3-NP treated wild-type cells and denoted glycolysis and lipid/sterol metabolism.