Comparing the DNA Hypermethylome with Gene Mutations in Human Colorectal Cancer (original) (raw)
Figure 4
Verification of the HCT116 Top Tier Hypermethylome
List of HCT116 candidate hypermethylated genes selected for verification of expression (by RT-PCR of HCT116 and DKO cells) and promoter methylation (by MSP of HCT116 and DKO cells) status. Gene descriptions are indicated on the left side of the panel and gene names are shown next to the PCR results. Water (RT-PCR and MSP), in vitro methylated DNA (for MSP), and actin beta (ACTB) were used as controls for each individual gene; a representative sample is shown. Green arrows identify genes that verified the array results, red arrows those that did not.