Comparing the DNA Hypermethylome with Gene Mutations in Human Colorectal Cancer (original) (raw)
Figure 6
Verification of the HCT116 Next Tier Hypermethylome
Genes were selected for verification of expression (by RT-PCR of HCT116 and DKO cells) and promoter methylation (by MSP of HCT116 and DKO cells) status. Gene names are indicated on the left side of the panel and gene abbreviations are shown next to the PCR results. Water (RT-PCR and MSP), in vitro methylated DNA (for MSP), and actin beta (ACTB) were used as controls for each individual gene; a representative sample is shown. Green arrows identify genes that verified the array results, red arrows those that did not as discussed in the text.