X Chromosome Activity in Mouse XX Primordial Germ Cells (original) (raw)
Figure 7
XX, but Not XY, UGRs Induce Xi Reactivation in E9.5 and E11.5 XX PGCs
A transwell system was used to study the X-chromosome reactivation in E9.5 and E11.5 XGFP PGCs cultured for a period of 48 hours.
(A) The percentage of X-linked GFP-expressing XX E9.5 PGCs after culture. Hindgut and the surrounding mesentery were dissociated and cultured in the top compartment with the lower compartment containing: medium only (control), one dissociated XX E11.5 UGR pair (XX), or one dissociated XY E11.5 UGR pair (XY). Red bars depict the median, n is the total number of transwell filter membranes (each containing 1.5× embryo) analysed. *, p < 0.05.
(B) The percentage of X-linked GFP-expressing XX E11.5 PGCs after culture. FACS-sorted PGCs (SSEA1-positive) were cultured in the top compartment with FACS-sorted XX (XX) or XY (XY) E11.5 UGR somatic tissue (SSEA1-negative). Red bars depict the median, n is the total number of embryos analysed.
(C,D) GFP-positive (white arrow) and GFP-negative (asterisk) Xp-XGFP E9.5 PGCs (SSEA1-positive) after culture.
(E–J) Representative dot-plots showing FACS-analysis of E13.5 genital ridges from individual XX (E,F), XGFPX (G), XGFPX Sry (H), XGFPY (I), and XGFPY Sry (J) embryos.
(K,L) The percentage of FACS-analysed GFP-expressing PGCs (K) and surrounding somatic tissue (L) in the genital ridges of individual XGFPX, XGFPX Sry, XGFPY, and XGFPY Sry E13.5 embryos. PGCs and somatic cells were respectively positive and negative for PECAM1. Red bars depict the median, n is the total number of embryos analysed.