GADD45A Does Not Promote DNA Demethylation (original) (raw)
Figure 6
GADD45A does not promote demethylation of endogenous LINE1 sequences in human cells.
HEK293 cells were transfected with pcDNA3.1 control vector or the pcDNA3.1-GADD45A expression vector. Forty-eight hours after transfection, genomic DNA was isolated and treated with sodium bisulfite. A. LINE1 promoter sequences were amplified and the PCR products were digested with HinfI, which cleaves only the methylated PCR products. B. The intensity of each cleavage product (bands 1–5) obtained from control or GADD45A-transfected cells was quantitated by densitometry. The result from three independent experiments is displayed (mean +/− S.D.).