Transcription and Chromatin Organization of a Housekeeping Gene Cluster Containing an Integrated β-Globin Locus Control Region (original) (raw)
Figure 5
Chromatin organization at 8C3/C4 in the presence of the LCR.
(A) Histone H3 acetylation at promoters (▴) and other regions (*) of 8C3/C4 in WT E14.5 fetal liver and brain as determined by ChIP-analysis. Enrichments were normalized to the Amylase promoter. (B) Histone H3 acetylation at 8C3/C4 in WT and transgenic strains in E14.5 fetal liver. Enrichments were normalized to the Amylase promoter. (C) Histone H3 lysine 4 tri-methylation at 8C3/C4 in WT and transgenic strains in E14.5 fetal liver. Enrichments were normalized to the endogenous β-major promoter. (D) Histone H3 occupancy at 8C3/C4 in WT and transgenic strains in E14.5 fetal liver. Enrichments were normalized to the endogenous β-major promoter. Error bars in all graphs represent standard error (SE) of at least two independent experiments.