FHY1 Mediates Nuclear Import of the Light-Activated Phytochrome A Photoreceptor (original) (raw)
Figure 7
Cytoplasmically localized FHY1 CT induces a dominant negative phenotype.
(A) Morphology of seedlings expressing FHY1 CT. Wild-type (L_er_), _fhy1_-1, and phyA_-201 seedlings as well as transgenic lines expressing different FHY1 167–202 ( = FHY1 CT) constructs in wild-type background were grown for 5 days in the dark or in FR (15 µmol m−2 s−1). #2590, #2619, #2643; L_er P35S_∶_YFP_-FHY1 167_–_202 (L_er YFP-FHY1 CT) #4520, #4527; L_er P35S_∶_NLS_-YFP_-FHY1 167_–_202 (L_er NLS-YFP-FHY1 CT, i.e. artificial FHY1) #4578, #4597; L_er P35S_∶_NES_-YFP_-FHY1 167_–_202 (L_er NES-YFP-FHY1 CT) (B) Cytoplasmically localized FHY1 CT inhibits phyA nuclear accumulation. L_er P35S_∶_NES_-_YFP_-FHY1 167_–_202 was crossed into _phyA_-_201 PPHYA_∶_PHYA_-CFP. F1 seedlings were grown for 3 days in the dark, irradiated for 6 h with FR (15 µmol m−2 s−1) and used for microscopic analysis. The scale bars represent 10 µm.