The Evolution of Epigenetic Regulators CTCF and BORIS/CTCFL in Amniotes (original) (raw)
Figure 4
Expression analysis of CTCF and BORIS.
(A) Conventional RT-PCR of CTCF and BORIS after 35 cycles. Note, cattle brain, platypus ovary and bearded dragon muscle could not be tested due to tissue unavailability or poor RNA quality. (B) BORIS transcript levels relative to the positive control gene GAPDH as quantified by real-time PCR. To assist comparisons between species, we set BORIS expression in the testis to 1 and adjusted all other values within the same species proportionally. As found in humans, expression of BORIS in somatic tissues of cattle and wallaby did not exceed 0.3% of that found in testis, although ovarian expression is just on or above this threshold. In contrast, levels of BORIS expression in platypus (liver and kidney) and bearded dragon (brain, kidney and ovary) are well above this threshold and in some cases match CTCF expression (Figure S2).