Drosophila Kismet Regulates Histone H3 Lysine 27 Methylation and Early Elongation by RNA Polymerase II (original) (raw)
Figure 10
Loss of TRX and ASH1 function also leads to increased H3K27 methylation.
The levels of H3K27me3 (A–D, red) on polytene chromosomes isolated from wild-type (A, C), ash122/ash117 (B) and trx1 (D) larvae were detected by double-label indirect immunofluorescence microscopy. H3K27me3 levels are higher on polytene chromosomes isolated from ash122/ash117 and trx1 mutants as compared to wild-type chromosomes. As an internal control, the chromosomes were simultaneously stained with antibodies against the RPB1 subunit of RNA Pol II (inset in lower right corner of A–D, green).