Adaptive Copy Number Evolution in Malaria Parasites (original) (raw)
Figure 2
Structure and gene content of gch1 amplicons.
The span of each amplicon was established by real-time PCR and breakpoint specific PCR assays. (a) Real-time PCR estimates of relative copy number plotted for 9 genes including gch1 on chr. 12. The error bars show 95% confidence intervals around the copy number estimate. The three plots show the profiles observed for the 2.3 kb (top), 7.3 kb (middle) and 8.7 kb (bottom) amplicons. The location of gch1 is marked by a red bar (b) Plot showing the span of the 5 amplicons types. The abundance of each of the amplicon types in the Thai sample is shown in white on the bars. The gene content in this region of the chr. 12 are shown beneath the graph: all breakpoints were between genes. The 5′ boundary for the >11 kb amplicon fell outside the range of our real-time PCR assays and was not defined: the bar shows the minimum size estimate for this amplicon.