Bidirectional Transcription Directs Both Transcriptional Gene Activation and Suppression in Human Cells (original) (raw)
Figure 2
Suppression of p21 antisense transcription results in a loss of suppressive H3K27me3 epigenetic mark at the p21 promoter.
The activating marks (H3K4 and H3K14) and the suppressive mark H3K27me3 were assessed by ChIP analysis in p21-322 and Bx332409 siRNA treated cells. (A) Schematic of the various p21-322 and Bx33409 siRNA target loci as well as primer sets (sets 1–3) used in the subsequent ChIP analysis are shown. The results from duplicate siRNA transfected cultures are shown with primer sets spanning (B) the p21-322 promoter target site (Set A), (C) in the coding region of p21, and (D) spanning the p21-322 and Bx332409 targeted site in the p21 mRNA. The standard error of the means is shown for quadruplicate measurements of the duplicate transfected cultures and the respective p values from paired T tests.