Topoisomerase 3α and RMI1 Suppress Somatic Crossovers and Are Essential for Resolution of Meiotic Recombination Intermediates in Arabidopsis thaliana (original) (raw)
Figure 4
Recombination frequencies of untreated and bleomycin treated T-DNA insertion lines.
Mean value of at least five independent assays using the IC9C background line in which restoration of the functional GUS-gene was only possible by intermolecular recombination. All three T-DNA insertion lines (recq4A-4, top3α-2 and rmi1-1) showed a five to seven-fold enhanced basic level of crossovers. The absolute numbers of blue sectors after induction of DSBs by bleomycin were more or less the same in all three lines. This indicates that the RTR complex is not involved in intermolecular DSB repair but rather in replication-dependent recombination.