Ploidy Reductions in Murine Fusion-Derived Hepatocytes (original) (raw)
Figure 2
Marker loss in livers repopulated with fusion-derived hepatocytes.
(A–D) Marker analysis was performed on sequential liver sections from serially transplanted mice. The transplantation scheme (F>M>F or M>F>F) is indicated. FAH+ nodules (brown) either co-expressed the Y-chromosome (black dots) (A) or were devoid of Y-chromosome staining (B and C). Double asterisks (**) indicate double positive nodules (FAH+ and Y-chromosome+) whereas single asterisks (*) indicate single positive nodules (FAH+ and Y-chromosome−). (D) ß-gal (blue staining) is expressed in a single FAH+ nodule but is absent from peripheral FAH+ tissue. Double diamonds (◊◊) indicate double positive tissue (FAH+ and ß-gal+) and single diamonds (◊) indicate a single positive nodule (FAH+ and ß-gal−). Scale bars are 200 µm. (E) Percentage of transplanted mice containing FAH+ fusion-derived nodules that lack Y-chromosome or ß-gal is shown.